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KMID : 0355319920150010081
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Journal of Korean Academy of Oral Pathology
1992 Volume.15 No. 1 p.81 ~ p.92
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MORPHOLOGIC STUDY OF MINERALIZATION IN PULP TRANSPLANTED INTO SUBCUTANEOUS TISSUE OF RAT
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1최홍란/1Hong-Ran Choi
2조규혁/2Kyu-Hyuk Cho
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Abstract
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-Abstract-
The denal pulp is the soft connective tissue that chiefly composed of odontoblasts,
fibroblasts, undifferentiated mesenchymal cells, and macrophages. The odontoblast is
important in the formation of dentin, and its functions similar to that of the osteoblast
in intramembranous bone formation. It was established that odontoblasts strongly
resemble to osteoblasts after calcification of dentin and, when cultured in vitro, they are
similar to each other morphologically and cytochemically. Bone and dentin are closely
related in both organic and inorganic composition.
The present study was undertaken to observe the morphologic changes of transplanted
pulp cells in rats.
The results were as follows :
1. Light microscopically, at the early stage(1-3days) of transplantation, hyalinized tissues
and calcospherites around of odontoblasts and fibroblasts of the transplanted tissue were
observed. Osteoid tissues and spongy bone surrounded by osteoblasts were observed at
the middle stage(5-11days). With the time lapsed, mineralization of the bones were
advanced.
More advanced mineralization in bone tissue was observed but osteoid tissues were not
observed at the late stage(2-4 weeks)
2. Electron microscopically, at the early stage of transplantation, the cell having eccentric
nucleus with abundant cytoplasm, well developed rough endoplasmic reticulum and round
shaped of mitochondria and resembled osteoblasts. A few of collagen fibers were
observed.
At the middle stage, osteoic and osteoblasts were observed. Osteoid tissues were
consist of irregularaly arranged of collagen fibers and osteoblasts disclosed well
developed rough endoplasmic reticulum with cisternal dilatations. Calcifying globules and
calcium deposits were observed in osteoid tissue.
From the above results, it is suggested that pulps tranasplanted subcutaneously, were
observed formed the bone tissues and as bone formations and maturations are various
according to the changes of environment and durations. It is though that odontoblasts,
fibroblasts and undifferentiated mesenchymal cells are invloved in bone formation.
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